Chemical antimutagensis in Escherichia coli mutator test systems.

by Marie Anne Flynn

Publisher: University of East Anglia in Norwich

Written in English
Published: Downloads: 877
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Edition Notes

Thesis (Ph.D.) - University of East Anglia, School of Biological Sciences, 1981.

ID Numbers
Open LibraryOL14512193M

All living organisms are continually exposed to agents that damage their DNA, which threatens the integrity of their genome. As a consequence, cells are equipped with a plethora of DNA repair enzymes to remove the damaged DNA. Unfortunately, situations nevertheless arise where lesions persist, and these lesions block the progression of the cell's replicase. Escherichia coli K RONALDH. HOESS* AND ROBERTK. HERMAN Department of Genetics and Cell Biology, University ofMinnesota, St. Paul, Minnesota Received for publication 30December Aselection procedure was devised to select for mutants of Escherichia coli K with enhanced rates of spontaneous frameshift mutation. Three types of. Product Specific References References for Escherichia coli antibody. Möller, J. et al. () Macrophages lift off surface-bound bacteria using a filopodium-lamellipodium hook-and-shovel mechanism. Sci Rep. 3: Raisch, J. et al. () Intracellular colon cancer-associated Escherichia coli promote protumoral activities of human macrophages by inducing sustained . Journal of Swine Health and Production —July and August, Tabl e 2: Genotypes of entero-toxigenic Escherichia coli strains isolated at the Animal Disease Research and Diagnostic Laboratory at South Dakota State University, April to April 1 Ages of pigs from which the strains were isolated are unknown.

Rabbit anti Escherichia coli antibody recognizes Escherichia coli and is broadly reactive with all somatic and capsular (O and K) antigenic serotypes. The somatic O antigens are composed of lipopolysaccharide complexes which form part of the cell wall structure of E. coli whilst the capsular K antigens are mainly composed of acidic polysaccharide. This antibody will remove . Fundamental Bacterial Genetics presents a concise introduction to microbial genetics. The text focuses on one bacterial species, Escherichia coli, but draws examples from other microbial systems at appropriate points to support the fundamental concepts of molecular genetics. A solid balance of concepts, techniques and applications makes this book an accessible, essential . One of the foundations of these procedures was the ingenious “Ames Test,” devised by Bruce Ames (see Ames et al., ) using the knowledge gained of repair in Escherichia coli to develop strains of the closely related Salmonella which would simultaneously detect a variety of types of chemical mutagen. Thousands of chemicals have been.   Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of .

Escherichia coli is one of the most widely used hosts for the production of heterologous proteins and its genetics are far better characterized than those of any other microorganism. Recent progress in the fundamental understanding of transcription, translation, and protein folding in E. coli.   DNA is a dynamic molecule that is constantly damaged and repaired. Major sources of DNA lesions are physical and chemical agents from the environment, intermediates of cellular metabolism, spontaneous chemical reactions of DNA, incorporation of foreign or damaged nucleotides, etc. [1,2].As a response to DNA damage, essentially all organisms have . In , Luria and Delbrück observed that cells resistant to viral infection appeared when a population of Escherichia coli bacteria was placed on agar plates containing a virus (a lytic phage). They examined the statistical distribution of resistant mutant cells and, on this basis (i.e., by performing what they called a “fluctuation test. @article{osti_, title = {In vitro characterization of pol I*, an SOS inducible, error-prone from of Escherichia coli DNA polymerase I}, author = {Hodes, C S}, abstractNote = {E. coli strains carrying mutations in the SOS regulon were screened for the presence of pol I*. Use of pol I* in assays of polymerase fidelity and selectivity has.

Chemical antimutagensis in Escherichia coli mutator test systems. by Marie Anne Flynn Download PDF EPUB FB2

Slupska M S, Baikalov C, Lloyd R, Miller J H. Mutator tRNAs are encoded by the Escherichia coli mutator genes mutA and mutC: a novel pathway for mutagenesis.

Proc Natl Acad Sci USA. ; – [PMC free article]. Mutators may present an enhanced risk for the emergence of antibiotic resistance in bacteria during chemotherapy.

Using Escherichia coli mutators as a model, we evaluated their ability to develop resistance to antibiotics routinely used for the treatment of urinary tract infections (UTIs). Under conditions that simulate therapeutic drug concentrations in humans, low-level Cited by: The isolation and characterization of Escherichia coli mutator genes have led to a better understanding of DNA replication fidelity mechanisms and to the discovery of important DNA repair pathways and their relationship to spontaneous mutagenesis.

Mutator strains in a population of cells can be beneficial in that they allow rapid selection of variants during periods Cited by:   Escherichia coli and other species of Escherichia ferment various sugars producing acid with gas.

But these sugar fermentation tests are of no diagnostic value in routine laboratory tests except sucrose fermentation test, However, that is also not well understood and some strains are able to ferment sucrose as well, but it can be used in differentiating Escherichia coli.

Mutation Research Elsevier Publishing Company, Amsterdam Printed in The Netherlands 35 CHEMICAL MUTAGENESIS IN E. COLI B/R; THE INFLUENCE OF REPAIR SYSTEMS FOR UV DAMAGE C.

CLARKE School of Biological Sciences, University of East Anglia, Norwich (Great Britain) (Received January I6th, ) SUMMARY Broth-grown cells of the Escherichia coli Cited by: Direct Selection for Mutators in Escherichia coli JEFFREY H.

MILLER,* ANJALI SUTHAR, JENNIFER TAI, ANNIE YEUNG, CINDY TRUONG, AND JEAN LEE STEWART Department of Microbiology and Molecular Genetics and The Molecular Biology Institute, University of California, Los Angeles, California Received 28 August /Accepted 8 December We demonstrate that this system can be used to evolve antibiotic resistance in wild-type E.

coli in chemical mutagens, ultraviolet light and the mutator. Abstract. An azaserine-resistant derivative of Escherichia coli B/UV, AZA/R 1, was found to carry a mutator gene, designated mutS1, was mapped by means of conjugation and P1kc-mediated mutS1 gene was cotransduced with argB at a frequency of %; the gene order in this region of the chromosome is thy argB determine whether a.

Our lake has been tested for E. Coli and the results were given as such: 1: > 2: 5. 3: 5. and 4: I assume they did 5 separate tests but did not explain if these are high or low and whether our is contaminated to the point that we cannot swim in or uuse its water to take showers or let the dogs in it since they drink the water.

Table of Contents hide Biochemical Test of Escherichia coli (E. coli) Fermentation of Enzymatic Reactions Biochemical Test of Escherichia coli (E. coli) Share the article on:Biochemical Test of Escherichia coli (E. coli) Basic Characteristics Properties (E.

coli) Capsule Capsulated Catalase Positive (+ve) Citrate Negative (-ve) Coagulase Negative (-ve). Escherichia coli detection for food and beverage safety: Rapid testing solutions and regulatory guidance to avoid contamination. According to the U.S. Center for Disease Control (CDC), the large and diverse bacterial group of gram-positive, rod shaped Escherichia coli, (E.

coli) contain numerous mammal-associated strains with many harmless, some beneficial, and some. The argE3 → Arg + reversion system in Escherichia coli K Escherichia coli K12 was isolated from the stool of a convalescent diphtheria patient in USA (Palo Alto, California) in and deposited in the strain collection of the Department of Bacteriology of Stanford University.

Serological studies revealed that after many years of cultivation under. In this system, a plasmid encoding full-length MvfR was mutagenized in the Escherichia coli XL1-Red mutator strain (Agilent Technologies, Inc.) (35), and the.

Abstract. The mutagenic and toxic effects of a series of N-alkyl-N'-nitro-N-nitrosoguanidines were examined in Escherichia coli K The role of nucleotide excision repair, the SOS response, and the adaptive response in both the reduction and the production of the biological effects of these chemicals was tested.

Escherichia coli: Uncovering Unknown # Jordyn Kidd. BIO oxygen requirements of the unknown (Lammert ). A GasPak anaerobic system that “uses a chemical reaction to consume free oxygen gas in a sealed jar” was then used in order to hydrolysis test was performed using an agar plate containing starch and was.

In Escherichia coli there are three specific proteins, called UvrA, B and C, involved in lesion recognition and endonuclease incision. An assembly of two UvrA and one UvrB protein binds to DNA nonspecifically; this assembly translocates unidirectionally in the genome, driven by the energy of ATP hydrolysis (Grossman and Yeung, ).

Mutator genesare that increase the mutation rate of other genes. They have been studied in Drosophila, maize, several strains of bacteria, and bacteri-ophage (1).

One such gene, the Treffers mutator gene of Escherichia coli, in-creases the mutation rate at least fold at most, if not all, chromosomal loci (2, 3). Because this mutator gene. After treatment of strainEscherichia coli AB with acridine orange and 5-bromouracil two temperature-sensitive mutants were isolated: AP 16 and AP These mutants were found to be sensitive and they formed revertants on treatment with N-methyl-N′-nitro-N-nitrosoguanidine, hydroxylamine, nitrous acid, sodium metabisulfite, methyl methanesulfonate, and proflavine.

Treffers mutator gene of E. coli K The effect ofthe mutator genes appears to be additive. Mutatorgenes havebeenfoundinthreelabora-tory strains of Escherichia coli (15, 35, 39). The genetic locations of two of these, the Treffers mutator gene ofK, and ast, the mutator gene ofthe Harvard strain, have beendetermined (36, 42).

The type of. COLI MUTATORGENE TABLE 4. Reversion ofargEin mutTJ/mutT+ diploidsa Strain Isolate no. Totalcells/ml arg+/ml Reversion frequency AB mutTI argErecA 1 X 1O X X 10r5 AB mutT+ argErecA+ 1 X 10' 0 mutator gene.

in gene mutT+ mutator mutator. Studies on the mutator gene, mutT of Escherichia coli. Molecular cloning of the gene, purification of the gene product, and identification of a novel nucleoside triphosphatase. J Biol Chem. Jun 25; (18)– Bhatnagar SK, Bullions LC, Bessman MJ. Characterization of the mutT nucleoside triphosphatase of Escherichia coli.

In vitro gene fusions that join an enzymatically active ß-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translation initiation signals, J.

Bacterial., () Google Scholar. The purpose of the study was to determine Escherichia coli and some physico-chemical parameters of selected pipe borne, sachet and bottled water in selected towns in Accra, Ghana.

Secondly, the study was conducted to make an alternate procedure to determine and test for bacteriological and chemical substances present in drinking water.

A selection procedure was devised to select for mutants of Escherichia coli K with enhanced rates of spontaneous frameshift mutation. Three types of mutants were isolated. Two of the mutations apparently represent alleles of previously isolated mutL13 and mutS3.

The third type of mutation, represented by two alleles, lies between lysA and thyA, and has been designated. A derivative of Escherichia coli K (strain ) has been developed in which mutations in several genes can be detected simultaneously by plating parts of the bacterial population on different selective mutation types include reversions from differently induced auxotrophies (nad- arg-) aand (forward) mutations leading to resistance against 5.

In the absence of pKM, Salmonella typhimurium appears to be less proficient than Escherichia coli in its capacity to carry out “error-prone repair”; but if pKM is present, both have similar capacities. In addition, much of this capacity to process chemical damage and give mutations is constitutively expressed in pKMcontaining.

Escherichia coli Mutators Present an Enhanced Risk for Emergence of Antibiotic Resistance during Urinary Tract Infections Article (PDF Available) in Antimicrobial Agents and. Glickman, B.

W., and M. Radman () Escherichia coli mutator mutants deficient in ire thylat ion ins true ted DNA mismatch correction.

Proc. Natl. Acad. Sci. USA –. Escherichia coli. Four different strains of Escherichia coli on Endo agar with biochemical slope.

Glucose fermentation with gas production, urea and H 2 S negative, lactose positive (with exception of strain D - "late lactose fermenter"; on Endo agar it looks like lactose negative).All four strains are mannitol positive (best seen in fig.

D), cellobiose negative (strains A, B). The Escherichia coli K SOS chromotest is a colorimetric (β-galactosidase induction) system for detecting genotoxic chemicals as agents which induce filamentation in response to DNA damage. The chromotest was modified from a liquid suspension assay to a simple, convenient agar spot test, which was performed in the manner of a related colorimetric.

Abstract. Mutagenesis by some alkylating agents and base analogs in Escherichia coli appears to involve a simple mispairing during replication. In contrast, mutagenesis by ultraviolet (UV) light and many chemicals in E. coli requires the participation of an inducible process that is dependent on the products of the umuD, umuC, and recA requirement for induced cellular.

BIOCHIMIE,64, CNRS symposium, May - TOULOUSE Inducible responses to DNA damages Effects of Escherichia coli mutator genes mutH, mutL and mutS on 2-aminopurine induced DNA repair. G. MAENHAUT-MICHEL and P. .Escherichia coli is the most common gram-negative mi-crobeisolated and identified in clinical microbiology labora-tories.

This laboratory previously reported a method for identifying E. coli strains within 1 h by oxidase, indole, lactose, and beta-glucuronidase tests (5, 6). The procedure was % specific and 93% sensitive. The oxidase and.